Anti-SEMA4A antibody (ab70178)
Key features and details
- Rabbit polyclonal to SEMA4A
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-SEMA4A antibody
See all SEMA4A primary antibodies -
Description
Rabbit polyclonal to SEMA4A -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total SEMA4A protein. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanAfrican green monkey -
Immunogen
A synthesized peptide derived from the internal region of human SEMA4A.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-SEMA4A antibody (ab70178) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
Lane 2 : Extracts from Jurkat cells
Lane 3 : Extracts from COS-7 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 117 kDa, 26 kDa. We are unsure as to the identity of these extra bands.
One other band detected greater then 117 kDa. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab70178 at 1/50-1/100 dilution.
Left image un-treated.Right image treated with immunizing peptide.