Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21963] to SCD1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SCD1 antibody [EPR21963] - BSA and Azide free
    See all SCD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21963] to SCD1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab238171 is recommended for human only in WB.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human adipose tissue within cardiac muscle tissue.

  • General notes

    Ab238171 is the carrier-free version of ab236868. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238171 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Western blot - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Western blot - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    All lanes : Anti-SCD1 antibody [EPR21963] (ab236868) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SCD knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 42 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab236868).

    anes 1-3: Merged signal (red and green). Green - ab236868. Red - loading control ab8245 observed at 50 kDa.

    ab236868 Anti-SCD1 antibody [EPR21963] was shown to specifically react with SCD1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265220 (knockout cell lysate ab257658) was used. Wild-type and SCD1 knockout samples were subjected to SDS-PAGE. ab236868 and Anti-tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunoprecipitation - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Immunoprecipitation - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

    SCD1 was immunoprecipitated from 0.35 mg of HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate with ab236868 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HEK-293 whole cell lysate 10 μg (input).
    Lane 2: ab236868 IP in HEK-293 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236868 in HEK-293 whole cell lysate (-).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 20876744; PMID: 9843580; PMID: 17449569). The full-length protein migrates at 37 kDa; the 28 kDa fragment may represent an SCD1 cleavage product.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236868).

  • Immunoprecipitation - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Immunoprecipitation - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

    SCD1 was immunoprecipitated from 10 μg of HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with ab236868 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (input).
    Lane 2: ab236868 IP in HepG2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236868 in HepG2 whole cell lysate (-).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    The expression profile observed is consistent with what has been described in the literature (PMID: 20876744; PMID: 9843580; PMID: 17449569). The full-length protein migrates at 37 kDa; the 28 kDa fragment may represent an SCD1 cleavage product.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236868).

  • Flow Cytometry - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Flow Cytometry - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling SCD1 with ab236868 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236868).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

    Immunohistochemical analysis of paraffin-embedded rat adipose tissue of pancreas tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in adipose cells in rat pancreas (PMID: 11500518) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236868).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

    Immunohistochemical analysis of paraffin-embedded mouse adipose tissue of stomach tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in adipose cells in mouse stomach (PMID: 11500518) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236868).

  • Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (Human malignant melanoma) cells labeling SCD1 with ab236868 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SK-MEL-28 cell line. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. Nuclear counterstain is DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236868).

  • Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling SCD1 with ab236868 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. Nuclear counterstain is DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236868).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

    Immunohistochemical analysis of paraffin-embedded human adipose tissue within cardiac muscle tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human adipose cells in cardiac muscle (PMID: 15907797) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab236868).

  • Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
    Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)

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