Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (ab251290)
![Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (ab251290)](https://www.abcam.com/ps/products/251/ab251290/Images/ab251290-405850-anti-phosphomevalonate-kinase-antibody-epr15029-bsa-and-azide-free-western-blot.jpg "Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (ab251290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15029] to Phosphomevalonate kinase - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free
See all Phosphomevalonate kinase primary antibodies -
Description
Rabbit monoclonal [EPR15029] to Phosphomevalonate kinase - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251290 is the carrier-free version of ab199725. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251290 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR15029 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (ab251290)All lanes : Anti-Phosphomevalonate kinase antibody [EPR15029] (ab199725) at 1/1000 dilution
Lane 1 : HEK293 (Human embryonic kidney) whole cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lane 5 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 3 minutesThis data was developed using ab199725, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (ab251290)](https://www.abcam.com/ps/products/251/ab251290/Images/ab251290-403272-anti-phosphomevalonate-kinase-antibody-epr15029-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (ab251290)All lanes : Anti-Phosphomevalonate kinase antibody [EPR15029] (ab199725) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 1 minuteThis data was developed using ab199725, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (ab251290)](https://www.abcam.com/ps/products/251/ab251290/Images/ab251290-3-benefits-of-recombinant-antibodies.png)
Anti-Phosphomevalonate kinase antibody [EPR15029] - BSA and Azide free (ab251290)