Human Apolipoprotein H ELISA Kit (APOH) (ab108814)
Key features and details
- Sensitivity: 0.45 ng/ml
- Range: 0.625 ng/ml - 40 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cell Lysate, Milk, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Apolipoprotein H ELISA Kit (APOH) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.2% Inter-assay Sample n Mean SD CV% Overall 10% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.45 ng/ml -
Range
0.625 ng/ml - 40 ng/ml -
Recovery
98 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Apolipoprotein H (APOH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein H concentrations in Human plasma, serum, saliva, urine, milk and cell culture samples.
An Apolipoprotein H specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein H specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein H captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Apolipoprotein H Antibody 1 x 120µl Apolipoprotein H Microplate (12 x 8 well strips) 1 unit Apolipoprotein H Standard (Lyophilized) 1 x 24ng Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Alternative names
- Activated protein C binding protein
- Activated protein C-binding protein
- Anticardiolipin cofactor
see all -
Database links
- SwissProt: P02749 Human
Images
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108814 - Sandwich elisa
Apolipoprotein H measured in biological fluids with background signal subtracted (duplicates +/- SD).The samples were tested in a range of dilutions (human serum and plasma at 1:10000-1:200000; milk at 1:100-1:1000 and the rest 1:1-1:50).
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Typical Standard CurveRepresentative Standard Curve using ab108814