Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [HL237] to SARS-CoV-2 Spike Glycoprotein S2 - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P, Sandwich ELISA

Overview

  • Product name

    Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free

  • Description

    Rabbit monoclonal [HL237] to SARS-CoV-2 Spike Glycoprotein S2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IHC-P, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Synthetic peptide within Human coronavirus SARS-CoV-2 Spike Glycoprotein S2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Carrier-protein conjugated synthetic peptide SARS-CoV-2 (strain Wuhan-Hu-1)

  • Positive control

    • IHC-P: SARS-CoV-2 (COVID-19) Spike transfected 293T cell ICC: SARS-CoV-2 (COVID-19) Spike transfected 293T cells. WB: SARS-CoV-2 (COVID-19) Spike transfected 293T cells. ELISA: Recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein.

Properties

Images

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV Spike transfected 293T whole cell extract
    Lane 3 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
    Lane 4 : MERS Spike transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)

    Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S2 stained (green) by ab281312 at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue).

    Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.

  • Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)

    Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike S2 stained (green) by ab281312 at 1/1000 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).

  • Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)

    Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag protein using a SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody as capture antibody at 5 µg/mL and ab281312 as detection antibody at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution and used to detect the primary antibody.

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S2 antibody [HL237] - BSA and Azide free (ab281312) at 1/5000 dilution

    Lane 1 : 293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    5% SDS-PAGE

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