Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624)
![Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624)](https://www.abcam.com/ps/products/277/ab277624/Images/ab277624-434726-AntiSpike-glycoprotein-antibody-2G1-ICC-HEK293-cells-transfected-with-DNA-encoding-the-Sprotein-segment.JPG "Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624)")
Key features and details
- Mouse monoclonal [2G1] to SARS-CoV-2 Spike Glycoprotein RBD
- Suitable for: WB, ICC
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1]
See all SARS-CoV-2 Spike Glycoprotein RBD primary antibodies -
Description
Mouse monoclonal [2G1] to SARS-CoV-2 Spike Glycoprotein RBD -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Recombinant fragment, SARS-CoV-2
Predicted to work with: Human coronavirus
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Immunogen
Recombinant fragment corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD.
Database link: P0DTC2 -
Positive control
- WB: HEK-293 cells transfected with the SARS-CoV2 binding domain. ICC: HEK-293 cells transfected with S-protein segment.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.07% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from Tissue Culture supernatant. -
Clonality
Monoclonal -
Clone number
2G1 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry - Anti-Spike glycoprotein antibody [2G1] (ab277624)
HEK-293 cells transfected with the DNA encoding the S-protein segment stained for spike glycoprotein using ab277624 (green) at 1/1000 dilution in Immunocytochemistry. The nuclei of transfected and untransfected cells are shown in blue with DAPI DNA stain.
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![Western blot - Anti-Spike glycoprotein antibody [2G1] (ab277624)](https://www.abcam.com/ps/products/277/ab277624/Images/ab277624-434725-AntiSpike-glycoprotein-antibody-2G1-WB-HEK293-cells-transfected-with-SARSCoV2-binding-domain.JPG)
Western blot - Anti-Spike glycoprotein antibody [2G1] (ab277624)Lanes 2-4 & 6-7 : Anti-SARS-CoV-2 Spike Glycoprotein RBD antibody [2G1] (ab277624) at 1/3000 dilution
Lanes 1 & 5 : Molecular weight ladder
Lane 2 : crude homogenate of untransfected control HEK-293 cells
Lane 3 : homogenate of HEK-293 cells expressing SARS-CoV2-bd
Lane 4 : homogenate of HEK-293 cells expressing GFP-SARS-CoV2-bd fusion
Lane 6 : full length recombinant SARS-CoV2 S-protein expressed in HEK293 at 1 µg
Lane 7 : full length recombinant SARS-CoV2 S-protein expressed in HEK293 at 0.1 µg
Additional bands at: 60 kDa (possible Loading Control)Western blot analysis of cells transfected with DNA encoding the S-protein ACE2 binding site in PROT-SARS-CoV2-bd which was inserted into pCI-Neo-Mod or pCI-Neo-GFP eukaryotic expression vectors, which express either the insert only or the insert fused with GFP using ab277624 (green) at 1/3000 dilution. The blot was also stained with rabbit polyclonal control antibody to HSP60 (red) at 1/5000 dilution.