Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)
![Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)](https://www.abcam.com/ps/products/281/ab281310/Images/ab281310-456645-SARSCoV2-nucleocapsid-protein-antibody-HL263-wb.jpg "Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL263] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
- Suitable for: WB, ICC, Indirect ELISA, IHC-P, Sandwich ELISA
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free
See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies -
Description
Rabbit monoclonal [HL263] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, Indirect ELISA, IHC-P, Sandwich ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant fragment within Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. SARS-CoV-2 (strain Wuhan-Hu-1)
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Positive control
- WB: SARS-CoV-2 Spike transfected 293T cells. ICC: SARS-CoV-2 Spike transfected 293T cells. IHC-P: SARS-CoV-2 Spike transfected 293T cells ELISA: Recombinant full-length SARS-CoV-2 spike protein, SARS-CoV-2 spike (full length) transfected 293T whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL263 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310) at 1/5000 dilution
Lane 1 : 293T whole cell extract
Lane 2 : SARS-CoV Spike transfected 293T whole cell extract
Lane 3 : SARS-CoV-2 (COVID-19) Spike transfected 293T whole cell extract
Lane 4 : MERS Spike transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
Lanes 1 & 3-4 : HRP-conjugated anti-rabbit IgG antibody
Lane 2 : The HRP-conjugated anti-rabbit IgG antibody
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)](https://www.abcam.com/ps/products/281/ab281310/Images/ab281310-458194-SARSCoV2-Spike-Glycoprotein-S1-antibody-HL263--elisa.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody as capture antibody at concentration of 5 μg/mL and ab281310 as detection antibody at concentration of 1 μg/mL. HRP-conjugated Rabbit IgG antibody was diluted at 1/10000 and used to detect the primary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - (ab281310)Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block. SARS-CoV-2 (COVID-19) Spike S1 stained by ab281310 (green) at 1/1000 dilution in immunohistochemical analysis. Fluoroshield with DAPI (blue). Antigen Retrieval was achieved with Citrate buffer at pH 6.0 for 15 minutes.
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![Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)](https://www.abcam.com/ps/products/281/ab281310/Images/ab281310-3-SARSCoV2-COVID19-Spike-S1-antibody-HL263-immunocytochemistry-293tcells.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)Mock and SARS-CoV-2 (COVID-19) Spike transfected 293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike stained by ab281310 (green) at 1/500 dilution in immunocytochemical analysis. Fluoroshield with DAPI (blue).
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![Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)](https://www.abcam.com/ps/products/281/ab281310/Images/ab281310-458193-SARSCoV2-Spike-Glycoprotein-S1-antibody-HL263--elisa2.JPG)
Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with ab281310 (2.4*10⁻⁶ ~ 20 nM). HRP-conjugated Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody.
EC50: 0.365 nM -
![Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)](https://www.abcam.com/ps/products/281/ab281310/Images/ab281310-2-SARSCoV2-COVID19-Spike-S1-antibody-HL263-indirectelisa.jpg)
Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 µg/mL to 4 µg/mL. The coated protein is detected with ab281310 at 1 µg/mL. HRP-conjugated Rabbit IgG antibody at 1/10000 dilution was used to detect the primary antibody.
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)](https://www.abcam.com/ps/products/281/ab281310/Images/ab281310-1-SARSCoV2-COVID19-Spike-S1-antibody-HL263-sandwichelisa.jpg)
Sandwich ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody as capture antibody at concentration of 5 µg/mL and ab281310 as detection antibody at concentration of 1 µg/mL. HRP-Rabbit IgG antibody was at 1/10000 dilution and used to detect the primary antibody.
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![Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)](https://www.abcam.com/ps/products/281/ab281310/Images/ab281310-4-SARSCoV2-COVID19-Spike-S1-antibody-HL263-westernblot-hek293tcells.jpg)
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310)All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [HL263] - BSA and Azide free (ab281310) at 1/5000 dilution
Lane 1 : 293T whole cell extract
Lane 2 : SARS-CoV-2 Spike transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody5% SDS-PAGE