HRV 3C Protease Inhibitor Assay Kit (Colorimetric) (ab211089)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Overview
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Product name
HRV 3C Protease Inhibitor Assay Kit (Colorimetric)
See all Human Rhinovirus 3C protease kits -
Detection method
Colorimetric -
Sample type
Inhibitor compounds -
Assay type
Quantitative -
Product overview
HRV 3C Protease Inhibitor Screening Kit (Colorimetric) (ab211089) provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of HRV 3C Protease inhibitors. The assay is based on the ability of a 3C Protease (derived from a HRV rhinovirus-14) to cleave a chromogenic peptide substrate to release a chromophore (pNA) which can be easily quantified using a microplate reader. In the presence of a HRV 3C Protease-specific inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the absorbance.
This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of HRV 3C Protease.
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Notes
Human rhinovirus (HRV) infections are the most frequent causative agents of common cold and various other upper respiratory tract infections. Rhinoviruses are members of the picornavirus family, which have a positive-sense, single-stranded RNA genome that is translated into a single polyprotein precursor. In the case of HRVs, the viral polyprotein is mainly processed by the proteases (2A and 3C) to generate functional proteins and enzymes.
Human rhinovirus 3C protease (EC: 3.4.22.28) is a cysteine protease that recognizes the cleavage site Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests HRV 3C Protease Assay Buffer 1 x 25ml HRV 3C Protease 2 x 100µl HRV 3C Protease Inhibitor (2 mM) 1 x 20µl HRV 3C Protease Substrate 1 x 500µl -
Research areas
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Relevance
Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.
Images
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HRV 3C Protease Inhibitor Screening Kit (Colorimetric) (ab211089)
Typical inhibition curve of HRV 3C protease activity by HRV 3C protease inhibitor (IC50 = 0.12 µM). Assay was performed following the kit protocol.