Anti-Human Rhinovirus 3C protease antibody (ab183574)
Key features and details
- Rabbit polyclonal to Human Rhinovirus 3C protease
- Suitable for: WB, IP
- Isotype: IgG
Overview
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Product name
Anti-Human Rhinovirus 3C protease antibody -
Description
Rabbit polyclonal to Human Rhinovirus 3C protease -
Host species
Rabbit -
Specificity
ab183574 recognizes the cleavage site of Human rhinovirus 3C protease. Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage. -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Other species -
Immunogen
Synthetic peptide corresponding to Human Rhinovirus 3C protease. Synthetic peptide recognizing the cleavage site of Human Rhinovirus 3C protease.
Database link: Q82081
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.01% BSA, 30% Glycerol, 69% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Human Rhinovirus 3C protease antibody (ab183574)All lanes : Anti-Human Rhinovirus 3C protease antibody (ab183574) at 1/1000 dilution
Lane 1 : Uncleaved human rhinovirus 3C protease
Lane 2 : Cleaved human rhinovirus 3C protease
Lysates/proteins at 1 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/15000 dilution
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Immunoprecipitation - Anti-Human Rhinovirus 3C protease antibody (ab183574)Immunoprecipitation of Human Rhinovirus 3C protease was performed on a control protein containing GST and the HRV3c cleavage site. Antigen-antibody complexes were formed by incubating 100ug HeLa lysate containing 2ug control protein with 4ug of ab183574 overnight on a rocking platform at 4°C. The immune complexes were captured on 50ul of Protein A/G Plus Agarose, washed extensively, and eluted with 5X Lane Marker Reducing Sample Buffer. Both the lysate and the immune complexes were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a GST monoclonal antibody at a dilution of 1:1500 overnight rotating at 4°C, washed in TBST, and probed with an IP detection reagent at a dilution of 1:2500 for at least 1 hour. Chemiluminescent detection was performed. Note: the IP fraction resulted in proteolytic activity of the GST-control protein.