Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19605] to Salivary alpha amylase - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free
    See all Salivary alpha amylase primary antibodies

  • Description

    Rabbit monoclonal [EPR19605] to Salivary alpha amylase - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This product reacts with Salivary alpha amylase (P04745), Pancreatic alpha amylase (P04746) and Carcinoid alpha amylase (P19961) due to high sequence homology.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251340 is the carrier-free version of ab201450. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251340 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    All lanes : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/10000 dilution

    Lane 1 : Human salivary alpha-amylase fragment recombinant protein
    Lane 2 : Human pancreatic alpha-amylase fragment recombinant protein
    Lane 3 : Human alpha-carcinoid alpha-amylase fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 58 kDa
    Observed band size: 22,22,50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab201450, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human salivary alpha-amylase contains aa311-511 with a GST-tag. Human pancreatic alpha-amylase and alpha-carcinoid alpha-amylase fragment recombinant proteins contain aa311-511 with a His-Tag®. These three recombinant proteins were made in-house.

  • Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Lanes 1-3 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/20000 dilution
    Lanes 4-6 : Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/1000 dilution

    Lane 1 : Human parotid gland lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal liver lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human fetal kidney lysate
    Lane 6 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab201450, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 3261213).

  • Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Western blot - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Anti-Salivary alpha amylase antibody [EPR19605] (ab201450) at 1/5000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab201450, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    This data was developed using ab201450, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human parotid gland tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human parotid gland is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    This data was developed using ab201450, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling salivary alpha-amylase + pancreatic alpha-amylase + alpha-carcinoid alpha-amylase with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    This data was developed using ab201450, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue with ab201450 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative control: No staining on human colon. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)
    Anti-Salivary alpha amylase antibody [EPR19605] - BSA and Azide free (ab251340)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.