Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14956] to P70 S6 Kinase beta/SRK - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free
    See all P70 S6 Kinase beta/SRK primary antibodies

  • Description

    Rabbit monoclonal [EPR14956] to P70 S6 Kinase beta/SRK - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250753 is the carrier-free version of ab184551.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)

    This data was developed using ab184551, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: RPS6KB2 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab184551 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab184551 was shown to specifically react with P70 S6 Kinase beta/SRK in wild-type HAP1 cells. No band was observed when RPS6KB2 knockout samples were used. Wild-type and RPS6KB2 knockout samples were subjected to SDS-PAGE. ab184551 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    Flow Cytometry - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    This data was developed using ab184551, the same antibody clone in a different buffer formulation.Flow cytometry analysis of 2% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/100 dilution (red) or a Rabbit monoclonal IgG (green). Goat anti rabbit IgG (FITC) secondary at a 1/150 dilution
  • Immunocytochemistry - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    Immunocytochemistry - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    This data was developed using ab184551, the same antibody clone in a different buffer formulation.Immunofluorescent staining of 4% paraformaldehyde fixed HeLa cells labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/250 dilution and a Goat anti rabbit IgG (Alexa Fluor® 488) secondary at a 1/200 dilution (green) costained with Dapi (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    This data was developed using ab184551, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas labeling P70 S6 Kinase beta/SRK using ab184551 at a 1/50 dilution, counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    All lanes : Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/50000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab184551, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    Western blot - Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - N-terminal (ab184551) at 1/10000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa



    This data was developed using ab184551, the same antibody clone in a different buffer formulation.

     

  • Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)
    Anti-P70 S6 Kinase beta/SRK antibody [EPR14956] - BSA and Azide free (ab250753)

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