Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)
![Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)](https://www.abcam.com/ps/products/250/ab250426/Images/ab250426-3-anti-sacsin-antibody-epr11864-61-bsa-and-azide-free-flow-cytometry.jpg "Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11864-61] to Sacsin - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free
See all Sacsin primary antibodies -
Description
Rabbit monoclonal [EPR11864-61] to Sacsin - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250426 is the carrier-free version of ab181207. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250426 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11864-61 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)This data was developed using ab181207, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling Sacsin with ab181207 at 1/470 dilution (red), or a rabbit IgG isotype control (green). Goat anti rabbit IgG (FITC) was used as a secondary antibody at 1/150 dilution.
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![Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)](https://www.abcam.com/ps/products/250/ab250426/Images/ab250426-403492-anti-sacsin-antibody-epr11864-61-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)All lanes : Anti-Sacsin antibody [EPR11864-61] (ab181207) at 1/5000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human glioma cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 521 kDaThis data was developed using ab181207, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)](https://www.abcam.com/ps/products/250/ab250426/Images/ab250426-398683-anti-sacsin-antibody-epr11864-61-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426) -
![Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)](https://www.abcam.com/ps/products/250/ab250426/Images/ab250426-4-benefits-of-recombinant-antibodies.png)
Anti-Sacsin antibody [EPR11864-61] - BSA and Azide free (ab250426)
