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Anti-MafB antibody [BLR046F] - BSA free (ab243902)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR046F] to MafB - BSA free
  • Suitable for: WB, ICC, IHC-P, IP
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-MafB antibody [BLR046F] - BSA free
    See all MafB primary antibodies

  • Description

    Rabbit monoclonal [BLR046F] to MafB - BSA free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MafB aa 1-50. The exact sequence is proprietary. NP_005452.2 and Gene ID 9935.
    Database link: Q9Y5Q3

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

Properties

Images

  • Immunoprecipitation - Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    Immunoprecipitation - Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    MafB was immunoprecipitated from 1.0 mg SK-N-MC whole cell lysate with ab243902 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243902 at 1/1000 dilution.

    Lane 1: ab243864 IP in SK-N-MC whole cell lysate.

    Lane 2: Control IgG in SK-N-MC whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 1 second.

  • Western blot - Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    Western blot - Anti-MafB antibody [BLR046F] - BSA free (ab243902)

    Detection of mouse MafB by western blot. Samples: Whole cell lysate (50 µg) from AlphaTC1 Clone 9 and Beta-TC-6 cells prepared using NETN lysis buffer using ab243902 at 1/1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH.

  • Western blot - Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    Western blot - Anti-MafB antibody [BLR046F] - BSA free (ab243902)

    Western blot analysis using ab243902 at 1/1000 dilution.

    Lane 1: HeLa cell whole cell lysate (50 µg).

    Lane 2: HEK-293T cell whole cell lysate (50 µg).

    Lane 3: SK-N-MC whole cell lysate (50 µg).

    Lane 4: RPMI 8226 whole cell lysate (50 µg).

    Lane 5: Jurkat whole cell lysate (50 µg).

    Lane 6: KG-1 whole cell lysate (50 µg).

    Lane 7: K562 whole cell lysate (50 µg).

    Lane 8: MOLT-4 whole cell lysate (50 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
  • Immunocytochemistry - Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    Immunocytochemistry - Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    Formalin-fixed, paraffin-embedded SK-N-MC cells labeling MafB using ab243902 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    Formalin-fixed, paraffin-embedded human pancreatic tumor tissue stained for MafB using ab243902 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
  • Anti-MafB antibody [BLR046F] - BSA free (ab243902)
    Anti-MafB antibody [BLR046F] - BSA free (ab243902)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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