Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [3/Jun] to c-Jun - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-c-Jun antibody [3/Jun] - BSA and Azide free
    See all c-Jun primary antibodies

  • Description

    Mouse monoclonal [3/Jun] to c-Jun - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa, NIH/3T3. PC-12 and MDA-MB-231 whole cell lysate. IHC-P: Human esophagus and colon tissue. Mouse and rat hippocampus tissue. Mouse and rat cerebrum tissue. ICC: MDA-MB-231 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab280094 is the carrier-free version of ab280089. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280094 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Western blot - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    All lanes : Anti-c-Jun antibody [3/Jun] (ab280089) at 1/1000 dilution

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
    Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 35 kDa



    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20976196).

    Negative control: T-47D (PMID: 24830720).

    Exposure time: lane 1: 3 minutes
    lane 2, 3:15 seconds
    lane 4: 37 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human esophagus.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Immunocytochemistry - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Immunocytochemistry - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling c-Jun with ab280089 at 1/50 (19.56 µg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).

    Confocal image showing nuclear staining in MDA-MB-231 cells.

    Negative control: MCF7 (PMID: 24830720).

    ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Negative control 1: ab280089 at a 1/50 dilution followed by ab150080 at a 1/500 dilution.

    Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.

  • Western blot - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Western blot - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    All lanes : Anti-c-Jun antibody [3/Jun] (ab280089) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 35 kDa



    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Lysates were made freshly and used in WB immediately to minimize protein degradation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: lane 1: 3 mins;

    lane 2, 3: 15 secs;

    lane 4: 37 secs.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Scattered nuclear staining on human colon.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of mouse hippocampus.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse cerebrum.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of rat hippocampus.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

    This data was developed using ab280089 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat cerebrum.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).

  • Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)
    Anti-c-Jun antibody [3/Jun] - BSA and Azide free (ab280094)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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