Anti-S100A9 antibody (ab81522)
Key features and details
- Goat polyclonal to S100A9
- Suitable for: WB, Flow Cyt, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-S100A9 antibody
See all S100A9 primary antibodies -
Description
Goat polyclonal to S100A9 -
Host species
Goat -
Tested applications
Suitable for: WB, Flow Cyt, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Cow
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Immunogen
Synthetic peptide:
C-DTNADKQLSFEEF
, corresponding to internal sequence amino acids 67-79 of Human S100A9 according to NP_002956.1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-S100A9 antibody (ab81522)
Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr at 10ug/mL, followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry - Anti-S100A9 antibody (ab81522)Immunocyohemistry analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10ug/mL, followed by Alexa Fluor 488 secondary antibody at 2ug/mL.
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Immunocytochemistry - Anti-S100A9 antibody (ab81522)Immunocytochemistry analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mlL.
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Western blot - Anti-S100A9 antibody (ab81522)All lanes : Anti-S100A9 antibody (ab81522) at 1 µg/ml
Lane 1 : Human bone marrow
Lane 2 : Gastrointestinal cancer lysate
Lane 3 : negative control HepG2 lysate.
Predicted band size: 13 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody (ab81522)Immunohistochemical analysis of paraffin embedded human liver tissue labelling S100A9 with ab81522 at 2.5μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A9 antibody (ab81522)Immunohistochemical analysis of paraffin embedded human lung tissue labelling S100A9 with ab81522 at 2.5μg/ml.
