Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-S100 beta antibody [4C4.9] (ab218513)

Key features and details

  • Mouse monoclonal [4C4.9] to S100 beta
  • Suitable for: SDS-PAGE, ICC, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-S100 beta antibody [4C4.9]
    See all S100 beta primary antibodies

  • Description

    Mouse monoclonal [4C4.9] to S100 beta

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) within Cow S100 beta aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (Purified from bovine brain).
    Database link: P02638

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human melanoma tissue. ICC: A2058 cells. WB: Human brain lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [4C4.9] (ab218513)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [4C4.9] (ab218513)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling S100 beta with ab218513 at 0.5 µg/mL.

  • Immunocytochemistry - Anti-S100 beta antibody [4C4.9] (ab218513)
    Immunocytochemistry - Anti-S100 beta antibody [4C4.9] (ab218513)

    Immunocytochemistry analysis of human A2058 cells labeling S100 beta with ab218513 at 1 ug/ml.

  • Western blot - Anti-S100 beta antibody [4C4.9] (ab218513)
    Western blot - Anti-S100 beta antibody [4C4.9] (ab218513)
    Anti-S100 beta antibody [4C4.9] (ab218513) at 1 µg/ml + Human brain lysate

    Predicted band size: 11 kDa

  • SDS-PAGE - Anti-S100 beta antibody [4C4.9] (ab218513)
    SDS-PAGE - Anti-S100 beta antibody [4C4.9] (ab218513)

    SDS-PAGE analysis of ab218513, loading 2 ug per lane. Non-reduced (Lane 1) and reduced (Lane 2).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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