Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21796] to Ryanodine Receptor
  • Suitable for: ICC/IF, WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ryanodine Receptor antibody [EPR21796]
    See all Ryanodine Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR21796] to Ryanodine Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle tissue lysate; mouse skeletal muscle tissue lysate; rat skeletal muscle tissue lysate. IHC-P: Mouse skeletal muscle and cerebellum tissue; rat skeletal muscle and cerebellum tissue. IHC-Fr: Mouse skeletal muscle and cerebellum tissue; rat skeletal muscle and cerebellum tissue. ICC/IF: C2C12 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Western blot - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    All lanes : Anti-Ryanodine Receptor antibody [EPR21796] (ab219798) at 1/1000 dilution

    Lane 1 : Human skeletal muscle tissue lysate at 20 µg
    Lane 2 : Mouse skeletal muscle tissue lysate at 20 µg
    Lane 3 : Rat skeletal muscle tissue lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 565 kDa
    Observed band size: 565 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 3: 3 minutes.

    Lane 2: 15 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of C2C12 (mouse muscle myoblast) cells labeling Ryanodine Receptor (green) with ab219798 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Confocal image showing cytoplasmic staining in differentiated C2C12 cells. Confluent C2C12 cells were grown for 8 days to differentiate into myotube in a complete culture medium containing 10% FBS, following the ATCC protocol for myotube formation. The nuclear counter stain is DAPI (blue).

    ab195889, Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Purkinje cells of mouse cerebellum (PMID 18313230) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse skeletal muscle tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Positive staining in mouse skeletal muscle cells (PMID: 21454501) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Purkinje cells of rat cerebellum (PMID 18313230) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in rat skeletal muscle (PMID: 26109061) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse skeletal muscle (PMID: 26109061) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Positive cytoplasmic staining in Purkinjie cells, negative staining in cells localized in granular layer and molecular layer of rat cerebellum tissue section (PMID: 18313230) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat skeletal muscle tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Positive staining of rat skeletal muscle cells (PMID: 21454501) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Positive cytoplasmic staining in the Purkinjie cells, negative staining in cells localized in granular layer and molecular layer of mouse cerebellum tissue section (PMID: 18313230) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

  • Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)
    Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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