Anti-RUNX1 / AML1 antibody (ab229482)
Key features and details
- Rabbit polyclonal to RUNX1 / AML1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-RUNX1 / AML1 antibody
See all RUNX1 / AML1 primary antibodies -
Description
Rabbit polyclonal to RUNX1 / AML1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human RUNX1/ AML1 (internal sequence). The exact sequence is proprietary.
Database link: Q01196 -
Positive control
- WB: RAW 264.7, C2C12, Rat2 and Jurkat whole cell extracts; Jurkat nuclear extract. ICC/IF: SK-N-SH cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Research areas
Images
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Western blot - Anti-RUNX1 / AML1 antibody (ab229482)All lanes : Anti-RUNX1 / AML1 antibody (ab229482) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Jurkat nuclear extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa10% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-RUNX1 / AML1 antibody (ab229482)SK-N-SH (human neuroblastoma cell line) cells stained for RUNX1 / AML1 (green) using ab229482 at 1/400 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue). The cytoskeleton is stained with Phalloidin (red).
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Western blot - Anti-RUNX1 / AML1 antibody (ab229482)All lanes : Anti-RUNX1 / AML1 antibody (ab229482) at 1/1000 dilution
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 2 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 48 kDa10% SDS-PAGE
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Western blot - Anti-RUNX1 / AML1 antibody (ab229482)Anti-RUNX1 / AML1 antibody (ab229482) at 1/1000 dilution + Rat2 (rat fibroblast cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 48 kDa10% SDS-PAGE
