Anti-RNF14 antibody [EPR9041] (ab134927)
![Anti-RNF14 antibody [EPR9041] (ab134927)](https://www.abcam.com/ps/products/134/ab134927/Images/ab134927-149097-anti-rnf14-antibody-epr9041-western-blot.jpg "Anti-RNF14 antibody [EPR9041] (ab134927)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9041] to RNF14
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-RNF14 antibody [EPR9041]
See all RNF14 primary antibodies -
Description
Rabbit monoclonal [EPR9041] to RNF14 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human RNF14 aa 350-450. The exact sequence is proprietary.
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Positive control
- LNCaP, K562, Jurkat, and HeLa whole cell lysate (ab150035). Permeabilized K562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 2.18 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9041 -
Isotype
IgG -
Research areas
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
Images
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Western blot - Anti-RNF14 antibody [EPR9041] (ab134927)All lanes : Anti-RNF14 antibody [EPR9041] (ab134927) at 1/1000 dilution
Lane 1 : LNCaP cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa
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![Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)](https://www.abcam.com/ps/products/134/ab134927/Images/ab134927-149096-anti-rnf14-antibody-epr9041-flow-cytometry.jpg)
Flow Cytometry - Anti-RNF14 antibody [EPR9041] (ab134927)Flow cytometric analysis of permeabilized K562 cells labelling RNF14 with ab134927 at 1/10 dilution (red) or a non-specific rabbit IgG (green). -
![OI-RD Scanning - Anti-RNF14 antibody [EPR9041] (ab134927)](https://www.abcam.com/ps/products/134/ab134927/Images/ab134927-149095-anti-rnf14-antibody-epr9041.jpg)
OI-RD Scanning - Anti-RNF14 antibody [EPR9041] (ab134927) -
![Anti-RNF14 antibody [EPR9041] (ab134927)](https://www.abcam.com/ps/products/134/ab134927/Images/ab134927-4-benefits-of-recombinant-antibodies.png)
Anti-RNF14 antibody [EPR9041] (ab134927)