Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CBL (phospho Y674) antibody [EPR2227] - BSA and Azide free (ab247486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2227] to CBL (phospho Y674) - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CBL (phospho Y674) antibody [EPR2227] - BSA and Azide free
    See all CBL primary antibodies

  • Description

    Rabbit monoclonal [EPR2227] to CBL (phospho Y674) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247486 is the carrier-free version of ab76536 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-CBL (phospho Y674) antibody [EPR2227] - BSA and Azide free (ab247486)
    Western blot - Anti-CBL (phospho Y674) antibody [EPR2227] - BSA and Azide free (ab247486)
    All lanes : Anti-CBL (phospho Y674) antibody [EPR2227] (ab76536) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate treated with pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size: 100 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76536, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-CBL (phospho Y674) antibody [EPR2227] - BSA and Azide free (ab247486)
    Flow Cytometry - Anti-CBL (phospho Y674) antibody [EPR2227] - BSA and Azide free (ab247486)
    This data was developed using ab76536, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells, un-treated (green) or pervanadate treated (red) using ab76536 at a 1/20 dilution, and pervanadate-treated Jurkat cells using the same antibody pre-incubated with phospho-CBL peptide (blue) or non-phospho-CBL peptide (orange).
  • Anti-CBL (phospho Y674) antibody [EPR2227] - BSA and Azide free (ab247486)
    Anti-CBL (phospho Y674) antibody [EPR2227] - BSA and Azide free (ab247486)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.