All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] (ab76292) at 1/200000 dilution

Lane 1 : Human brain tissue lysate, untreated
Lane 2 : Human brain tissue lysate treated with AP

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

Predicted band size: 217 kDa
Observed band size: 270 kDa why is the actual band size different from the predicted?

This data was developed using ab76292, the same antibody clone in a different buffer formulation.

This data was developed using ab76292, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells (untreated and treated with AP) labelling RNA polymerase II CTD repeat YSPTSPS (phospho S1801) with ab76292 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used. Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000). Decreased nuclear staining can be seen on SH-SY5Y cells after AP treatment.

This data was developed using ab76292, the same antibody clone in a different buffer formulation.ab76292, at a 1/100 dilution, staining RNA Polymerase II (phospho S1801) in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

This data was developed using ab76292, the same antibody clone in a different buffer formulation.ab76292, at a 1/250 dilution, staining RNA Polymerase II (phospho S1801) in HeLa cells by Immunofluorescence.

This data was developed using ab76292, the same antibody clone in a different buffer formulation.Dot blot analysis of RNA polymerase II (pS1801) peptide (Lane 1) and RNA polymerase II non-phospho peptide (Lane 2) labelling RNA polymerase (phospho S1801) with ab76292 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 10 seconds.