Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1510Y] to RNA polymerase II CTD repeat YSPTSPS (phospho S5) - BSA and Azide free
  • Suitable for: ICC, Dot blot, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free
    See all RNA polymerase II CTD repeat YSPTSPS primary antibodies

  • Description

    Rabbit monoclonal [EP1510Y] to RNA polymerase II CTD repeat YSPTSPS (phospho S5) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Detects RNA Polymeraste II phosphorylated at serine 5.

  • Tested applications

    Suitable for: ICC, Dot blot, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247458 is the carrier-free version of ab76292. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247458 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    All lanes : Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] (ab76292) at 1/200000 dilution

    Lane 1 : Human brain tissue lysate, untreated
    Lane 2 : Human brain tissue lysate treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

    Predicted band size: 217 kDa
    Observed band size: 270 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76292, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    Immunocytochemistry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    This data was developed using ab76292, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells (untreated and treated with AP) labelling RNA polymerase II CTD repeat YSPTSPS (phospho S1801) with ab76292 at a dilution of 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used. Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000). Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000). Decreased nuclear staining can be seen on SH-SY5Y cells after AP treatment.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    This data was developed using ab76292, the same antibody clone in a different buffer formulation.ab76292, at a 1/100 dilution, staining RNA Polymerase II (phospho S1801) in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    Immunocytochemistry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    This data was developed using ab76292, the same antibody clone in a different buffer formulation.ab76292, at a 1/250 dilution, staining RNA Polymerase II (phospho S1801) in HeLa cells by Immunofluorescence.
  • Dot Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    Dot Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    This data was developed using ab76292, the same antibody clone in a different buffer formulation.Dot blot analysis of RNA polymerase II (pS1801) peptide (Lane 1) and RNA polymerase II non-phospho peptide (Lane 2) labelling RNA polymerase (phospho S1801) with ab76292 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)
    Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [EP1510Y] - BSA and Azide free (ab247458)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.