Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RhoGAP antibody [EP489Y] (ab32328)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP489Y] to RhoGAP
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RhoGAP antibody [EP489Y]
    See all RhoGAP primary antibodies

  • Description

    Rabbit monoclonal [EP489Y] to RhoGAP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human RhoGAP aa 1150-1250. The exact sequence is proprietary.

  • Positive control

    • Human stomach carcinoma and the following cell lysates: Hela, NIH 3T3, Jurkat, 293, K562, MCF-7, A431 and PC12.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP489Y

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-RhoGAP antibody [EP489Y] (ab32328)
    Flow Cytometry - Anti-RhoGAP antibody [EP489Y] (ab32328)
    Overlay histogram showing A431 cells stained with ab32328 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32328, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328)
    Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328) This image is courtesy of an anonymous Abreview
    Anti-RhoGAP antibody [EP489Y] (ab32328) at 1/500 dilution + Mouse MEFs whole cell lysate at 50 µg

    Secondary
    HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 171 kDa
    Observed band size: 190 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328)
    Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328)
    All lanes : Anti-RhoGAP antibody [EP489Y] (ab32328) at 1/500 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : A431 cell lysate
    Lane 6 : 293 cell lysate
    Lane 7 : NIH 3T3 cell lysate
    Lane 8 : PC12 cell lysate

    Predicted band size: 171 kDa
    Observed band size: 190 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGAP antibody [EP489Y] (ab32328)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGAP antibody [EP489Y] (ab32328)

    ab32328, at a 1/100 dilution, staining human RhoGAP in stomach carcinoma by Immunohistochemistry, Paraffin embedded tissue

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-RhoGAP antibody [EP489Y] (ab32328)
    Anti-RhoGAP antibody [EP489Y] (ab32328)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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