Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP489Y] to RhoGAP
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-RhoGAP antibody [EP489Y]
See all RhoGAP primary antibodies
Description

Rabbit monoclonal [EP489Y] to RhoGAP
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human RhoGAP aa 1150-1250. The exact sequence is proprietary.
Positive control
- Human stomach carcinoma and the following cell lysates: Hela, NIH 3T3, Jurkat, 293, K562, MCF-7, A431 and PC12.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP489Y
Isotype

IgG
Research areas

Flow Cytometry - Anti-RhoGAP antibody [EP489Y] (ab32328)

Overlay histogram showing A431 cells stained with ab32328 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32328, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328) This image is courtesy of an anonymous Abreview

Anti-RhoGAP antibody [EP489Y] (ab32328) at 1/500 dilution + Mouse MEFs whole cell lysate at 50 µg

Secondary
HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/2000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 171 kDa
Observed band size: 190 kDa
why is the actual band size different from the predicted?

Exposure time: 5 minutes
See Abreview
Western blot - Anti-RhoGAP antibody [EP489Y] (ab32328)

All lanes : Anti-RhoGAP antibody [EP489Y] (ab32328) at 1/500 dilution

Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysate
Lane 4 : K562 cell lysate
Lane 5 : A431 cell lysate
Lane 6 : 293 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : PC12 cell lysate

Predicted band size: 171 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGAP antibody [EP489Y] (ab32328)

ab32328, at a 1/100 dilution, staining human RhoGAP in stomach carcinoma by Immunohistochemistry, Paraffin embedded tissue

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-RhoGAP antibody [EP489Y] (ab32328)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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