Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Wednesday March 03, 2021

Anti-RBM47 antibody [EPR9658] (ab167164)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9658] to RBM47
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-RBM47 antibody [EPR9658]
See all RBM47 primary antibodies
Description

Rabbit monoclonal [EPR9658] to RBM47
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues in Human RBM47 (UniProt A0AV96).
Positive control
- A549, Caco-2, A431, NIH/3T3 and Raw264.7 cell lysates; Human breast carcinoma and colon tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9658
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (ab167164)

ab167164 showing +ve staining in Human normal uterus.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (ab167164)

ab167164 showing +ve staining in Human thyroid gland .

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (ab167164)

ab167164 showing +ve staining in Human prostate hyperplasia.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (ab167164)

ab167164 showing +ve staining in Human lung carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-RBM47 antibody [EPR9658] (ab167164)

All lanes : Anti-RBM47 antibody [EPR9658] (ab167164) at 1/1000 dilution

Lane 1 : A549 cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : Raw264.7 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (ab167164)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling RBM47 with ab167164 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM47 antibody [EPR9658] (ab167164)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling RBM47 with ab167164 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-RBM47 antibody [EPR9658] (ab167164)

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