Anti-RALY antibody [EPR10121] (ab170105)
![Anti-RALY antibody [EPR10121] (ab170105)](https://www.abcam.com/ps/products/170/ab170105/Images/ab170105-170044-anti-raly-antibody-epr10121-western-blot.jpg "Anti-RALY antibody [EPR10121] (ab170105)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10121] to RALY
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-RALY antibody [EPR10121]
See all RALY primary antibodies -
Description
Rabbit monoclonal [EPR10121] to RALY -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human RALY (UniProt: Q9UKM9).
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Positive control
- Recombinant Human RALY protein (ab161475) can be used as a positive control in WB. HeLa, HepG2, Jurkat and A549 lysates; Human brain and heart tissues; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10121 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RALY antibody [EPR10121] (ab170105)All lanes : Anti-RALY antibody [EPR10121] (ab170105) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALY antibody [EPR10121] (ab170105)](https://www.abcam.com/ps/products/170/ab170105/Images/ab170105-170043-anti-raly-antibody-epr10121-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALY antibody [EPR10121] (ab170105)Immunohistochemical analysis of paraffin embedded Human brain tissue labeling RALY with ab170105 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALY antibody [EPR10121] (ab170105)](https://www.abcam.com/ps/products/170/ab170105/Images/ab170105-170042-anti-raly-antibody-epr10121-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RALY antibody [EPR10121] (ab170105)Immunohistochemical analysis of paraffin embedded Human heart tissue labeling RALY with ab170105 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-RALY antibody [EPR10121] (ab170105)](https://www.abcam.com/ps/products/170/ab170105/Images/ab170105-170041-anti-raly-antibody-epr10121-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RALY antibody [EPR10121] (ab170105)Immunofluorescence analysis of HepG2 cells labeling RALY with ab170105 at a 1/250 dilution. -
![Anti-RALY antibody [EPR10121] (ab170105)](https://www.abcam.com/ps/products/170/ab170105/Images/ab170105-5-benefits-of-recombinant-antibodies.png)
Anti-RALY antibody [EPR10121] (ab170105)
