Anti-RAVER2 antibody [EPR12601] (ab174321)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12601] to RAVER2
- Suitable for: ICC/IF, IP, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-RAVER2 antibody [EPR12601]
See all RAVER2 primary antibodies -
Description
Rabbit monoclonal [EPR12601] to RAVER2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human RAVER2.
Database link: Q9HCJ3 -
Positive control
- Human fetal lung, HT1080, 293T, Human fetal brain, and Jurkat lysates; Human cervical carcinoma and tonsil tissues; 293T cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12601 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RAVER2 antibody [EPR12601] (ab174321) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : HT1080 cell lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal brain lysate
Lane 5 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
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Immunofluorescent analysis of 293T cells labeling RAVER2 with ab174321 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RAVER2 with ab174321 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling RAVER2 with ab174321 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from fetal lung lysate, immunoprecipitated using ab174321 at 1/10 dilution.
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