Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RAVER2 antibody [EPR12601] (ab174321)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12601] to RAVER2
Suitable for: ICC/IF, IP, IHC-P, WB
Reacts with: Human

Product name

Anti-RAVER2 antibody [EPR12601]
See all RAVER2 primary antibodies
Description

Rabbit monoclonal [EPR12601] to RAVER2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human RAVER2.
Database link: Q9HCJ3
Positive control
- Human fetal lung, HT1080, 293T, Human fetal brain, and Jurkat lysates; Human cervical carcinoma and tonsil tissues; 293T cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12601
Isotype

IgG
Research areas

Western blot - Anti-RAVER2 antibody [EPR12601] (ab174321)

All lanes : Anti-RAVER2 antibody [EPR12601] (ab174321) at 1/1000 dilution

Lane 1 : Human fetal lung lysate
Lane 2 : HT1080 cell lysate
Lane 3 : 293T lysate
Lane 4 : Human fetal brain lysate
Lane 5 : Jurkat lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 74 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RAVER2 antibody [EPR12601] (ab174321)

Immunofluorescent analysis of 293T cells labeling RAVER2 with ab174321 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAVER2 antibody [EPR12601] (ab174321)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling RAVER2 with ab174321 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAVER2 antibody [EPR12601] (ab174321)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling RAVER2 with ab174321 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunoprecipitation - Anti-RAVER2 antibody [EPR12601] (ab174321)

Western blot analysis on immunoprecipitation pellet from fetal lung lysate, immunoprecipitated using ab174321 at 1/10 dilution.
Anti-RAVER2 antibody [EPR12601] (ab174321)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com