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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Regulators

Anti-RASA4 antibody [EPR10515] (ab157190)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-RASA4 antibody [EPR10515] (ab157190)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10515] to RASA4
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-RASA4 antibody [EPR10515]
    See all RASA4 primary antibodies
  • Description

    Rabbit monoclonal [EPR10515] to RASA4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide, corresponding to residues in Human RASA4 (UniProt: O43374).

  • Positive control

    • Human skeletal muscle, HepG2, 293T and HeLa cell lysates; Human brain and skeletal muscle tissues.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10515
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)

    ab157190 showing +ve staining in Mouse kidney.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)

    ab157190 showing +ve staining in Human thyroid gland carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)

    ab157190 showing +ve staining in Human ovarian carcinoma.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)

    ab157190 showing +ve staining in Human heart.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-RASA4 antibody [EPR10515] (ab157190)
    Western blot - Anti-RASA4 antibody [EPR10515] (ab157190)
    All lanes : Anti-RASA4 antibody [EPR10515] (ab157190) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : HepG2 lysate
    Lane 3 : 293T lysate
    Lane 4 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 90 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RASA4 with ab157190 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RASA4 antibody [EPR10515] (ab157190)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling RASA4 with ab157190 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-RASA4 antibody [EPR10515] (ab157190)
    Anti-RASA4 antibody [EPR10515] (ab157190)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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