Anti-RASA4 antibody [EPR10515] (ab157190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10515] to RASA4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-RASA4 antibody [EPR10515]
See all RASA4 primary antibodies -
Description
Rabbit monoclonal [EPR10515] to RASA4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB Human -
Immunogen
Synthetic peptide, corresponding to residues in Human RASA4 (UniProt: O43374).
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Positive control
- Human skeletal muscle, HepG2, 293T and HeLa cell lysates; Human brain and skeletal muscle tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10515 -
Isotype
IgG -
Research areas
Images
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ab157190 showing +ve staining in Mouse kidney.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab157190 showing +ve staining in Human thyroid gland carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab157190 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab157190 showing +ve staining in Human heart.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-RASA4 antibody [EPR10515] (ab157190) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : HepG2 lysate
Lane 3 : 293T lysate
Lane 4 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 90 kDa
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RASA4 with ab157190 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling RASA4 with ab157190 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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