Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CPSF73 antibody [EPR7468] (ab131245)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7468] to CPSF73
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CPSF73 antibody [EPR7468]
    See all CPSF73 primary antibodies

  • Description

    Rabbit monoclonal [EPR7468] to CPSF73

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CPSF73 aa 650-750. The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, 293T, Jurkat, Caco-2, RAW 264.7, PC-12, and NIH/3T3 cell lysates. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)
    Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)
    All lanes : Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : Caco-2 cell lysate
    Lane 5 : RAW 264.7 cell lysate
    Lane 6 : PC-12 cell lysate
    Lane 7 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 77 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] (ab131245)
    Immunocytochemistry/ Immunofluorescence - Anti-CPSF73 antibody [EPR7468] (ab131245)
    Immunofluorescence analysis of HeLa cells labelling CPSF73 with ab131245 at 1/50 dilution.
  • Flow Cytometry - Anti-CPSF73 antibody [EPR7468] (ab131245)
    Flow Cytometry - Anti-CPSF73 antibody [EPR7468] (ab131245)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CPSF73 (red) with ab131245 at a 1/250 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)
    Western blot - Anti-CPSF73 antibody [EPR7468] (ab131245)
    Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilution + U2OS whole cell lysate at 100 µg

    Predicted band size: 77 kDa

    See Abreview

  • OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] (ab131245)
    OI-RD Scanning - Anti-CPSF73 antibody [EPR7468] (ab131245)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CPSF73 antibody [EPR7468] (ab131245)
    Anti-CPSF73 antibody [EPR7468] (ab131245)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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