Anti-Ras (mutated Q61) antibody [EPR20278] (ab222516)
![Anti-Ras (mutated Q61) antibody [EPR20278] (ab222516)](https://www.abcam.com/ps/products/222/ab222516/Images/ab222516-282916-anti-nras-mutated-q61-antibody-epr20278-western-blot.jpg "Anti-Ras (mutated Q61) antibody [EPR20278] (ab222516)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20278] to Ras (mutated Q61)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Ras (mutated Q61) antibody [EPR20278]
See all Ras primary antibodies -
Description
Rabbit monoclonal [EPR20278] to Ras (mutated Q61) -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 95% homology with NRAS, KRAS and HRAS.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SK-MEL-2 and HT-1197 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20278 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ras (mutated Q61) antibody [EPR20278] (ab222516)All lanes : Anti-Ras (mutated Q61) antibody [EPR20278] (ab222516) at 1/1000 dilution
Lane 1 : SK-MEL-2 (human skin malignant melanoma cell line) whole cell lysate
Lane 2 : HT-1197 (human urinary bladder carcinoma cell line) whole cell lysate
Lane 3 : MeWo (human malignant melanoma fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 21 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution: 5% NFDM/TBST.
SK-MEL-2 cell line contains N-Ras Q61R homozygous mutation. HT-1197 cell line contains N-Ras Q61R heterozygous mutation. MeWo cell line is wild-type for N-Ras.
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![Anti-Ras (mutated Q61) antibody [EPR20278] (ab222516)](https://www.abcam.com/ps/products/222/ab222516/Images/ab222516-2-benefits-of-recombinant-antibodies.png)
Anti-Ras (mutated Q61) antibody [EPR20278] (ab222516)
