Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)
![Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)](https://www.abcam.com/ps/products/227/ab227658/Images/ab227658-3-anti-nras-mutated-q64r-antibody-sp174-flowcytometry-skmel2-human.jpg "Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP174] to NRAS (mutated Q61R)
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-NRAS (mutated Q61R) antibody [SP174]
See all NRAS primary antibodies -
Description
Rabbit monoclonal [SP174] to NRAS (mutated Q61R) -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 91% homology with KRAS and HRAS. Cross-reactivity with these proteins has not been confirmed experimentally.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SK-MEL-2 cell lysate. IHC-P: SK-MEL-2 cells. Flow Cyt: SK-MEL-2
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP174 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)
Flow Cytometry analysis of SK-MEL-2( Human skin malignant melanoma) cells labeling NRAS with purifiedab227658 at 1:200 dilution (0.8 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
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![Western blot - Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)](https://www.abcam.com/ps/products/227/ab227658/Images/ab227658-290476-anti-nras-mutated-q61-r-antibody-sp174-western-blot.jpg)
Western blot - Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658) at 1/400 dilution + SK-MEL-2 cell lysate
Predicted band size: 21 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)](https://www.abcam.com/ps/products/227/ab227658/Images/ab227658-290475-anti-nras-mutated-q61-r-antibody-sp174-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)Formalin-fixed, paraffin-embedded human SK-MEL-2 cells stained for NRAS (mutated Q61R) using ab227658 at 1/100 dilution in immunohistochemical analysis.
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![Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)](https://www.abcam.com/ps/products/227/ab227658/Images/ab227658-4-benefits-of-recombinant-antibodies.png)
Anti-NRAS (mutated Q61R) antibody [SP174] (ab227658)
