Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)
![Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)](https://www.abcam.com/ps/products/267/ab267493/Images/ab267493-388408-antirab8aphosphos111antibodymjfr2730westernblotwildtypehelawildtypehelarab8aknockouthelarab8aknockouthelalysates.jpg "Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R27-30] to RAB8A (phospho S111) - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free
See all RAB8A primary antibodies -
Description
Rabbit monoclonal [MJF-R27-30] to RAB8A (phospho S111) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HeLa (treated with 10 uM CCCP), whole cell lysate.
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General notes
ab267493 is the carrier-free version of ab267492. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab267493 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R27-30 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)All lanes : Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492) at 1/1000 dilution
Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO
Lane 2 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP
Lane 3 : RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO
Lane 4 : RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 24 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
CCCP (carbonyl cyanide m-chlorophenyl hydrazone) inhibits oxidative phosphorylation.
The lysates were kindly provided by Pawan Singh Ph.D, University of Dundee.
Exposure time: 3min
This data was developed using the same antibody clone in a different buffer formulation containing PBS, glycerol, BSA and sodium azide (ab276492).
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![Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)](https://www.abcam.com/ps/products/267/ab267493/Images/ab267493-2-benefits-of-recombinant-antibodies.png)
Anti-RAB8A (phospho S111) antibody [MJF-R27-30] - BSA and Azide free (ab267493)
