Anti-RAB35 antibody (ab152138)
Key features and details
- Rabbit polyclonal to RAB35
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RAB35 antibody
See all RAB35 primary antibodies -
Description
Rabbit polyclonal to RAB35 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-201 of Human RAB35.
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Positive control
- WB: U-87 MG, SK-N-SH, IMR32, SK-N-AS, transfected HEK-293T cells and mouse brain tissue lysate; IHC-P: Human breast cancer tissue; IF: U87-MG whole cell.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-RAB35 antibody (ab152138) at 1/3000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
Lane 3 : IMR32 (human brain neuroblast cell line) whole cell lysate
Lane 4 : SK-N-AS (human brain neuroblastoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining RAB35 with ab152138 at 1/250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Immunofluorescence analysis of 4% paraformaldehyde-fixed U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell labeling RAB35 with ab152138 at 1/500 dilution. Blue: Hoechst 33342 staining.
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Anti-RAB35 antibody (ab152138) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE
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All lanes : Anti-RAB35 antibody (ab152138) at 1/2000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE