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Anti-RAB35 antibody (ab152138)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-RAB35 antibody (ab152138)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RAB35
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RAB35 antibody
    See all RAB35 primary antibodies
  • Description

    Rabbit polyclonal to RAB35
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-201 of Human RAB35.

  • Positive control

    • WB: U-87 MG, SK-N-SH, IMR32, SK-N-AS, transfected HEK-293T cells and mouse brain tissue lysate; IHC-P: Human breast cancer tissue; IF: U87-MG whole cell.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Images

  • Western blot - Anti-RAB35 antibody (ab152138)
    Western blot - Anti-RAB35 antibody (ab152138)
    All lanes : Anti-RAB35 antibody (ab152138) at 1/3000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell lysate
    Lane 3 : IMR32 (human brain neuroblast cell line) whole cell lysate
    Lane 4 : SK-N-AS (human brain neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23 kDa



    12% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB35 antibody (ab152138)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB35 antibody (ab152138)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining RAB35 with ab152138 at 1/250 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB35 antibody (ab152138)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB35 antibody (ab152138)

    Immunofluorescence analysis of  4% paraformaldehyde-fixed U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell labeling RAB35 with ab152138 at 1/500 dilution. Blue: Hoechst 33342 staining.

  • Western blot - Anti-RAB35 antibody (ab152138)
    Western blot - Anti-RAB35 antibody (ab152138)
    Anti-RAB35 antibody (ab152138) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg

    Secondary
    HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23 kDa



    12% SDS-PAGE

  • Western blot - Anti-RAB35 antibody (ab152138)
    Western blot - Anti-RAB35 antibody (ab152138)
    All lanes : Anti-RAB35 antibody (ab152138) at 1/2000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Transfected HEK-293T whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 23 kDa



    12% SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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