Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)
![Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)](https://www.abcam.com/ps/products/109/ab109462/Images/ab109462-122346-anti-pts-phospho-s19-antibody-epr15172-western-blot.jpg "Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1517(2)] to PTS/PTPS (phospho S19)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)]
See all PTS/PTPS primary antibodies -
Description
Rabbit monoclonal [EPR1517(2)] to PTS/PTPS (phospho S19) -
Host species
Rabbit -
Specificity
ab109462 only detects PTS/PTPS phosphorylated at Serine 19.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HUVEC cell lysates; Human brain tissue; HeLa cells
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as PTS
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1517(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)All lanes : Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462) at 1/1000 dilution
Lane 1 : HUVEC cell lysates
Lane 2 : HUVEC cell lysates treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)](https://www.abcam.com/ps/products/109/ab109462/Images/ab109462-122345-anti-pts-phospho-s19-antibody-epr15172-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)ab109462, at a 1/250 dilution, staining PTS/PTPS (phospho S19) in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)](https://www.abcam.com/ps/products/109/ab109462/Images/ab109462-122344-anti-pts-phospho-s19-antibody-epr15172-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)ab109462, at a 1/250 dilution, staining PTS/PTPS (phospho S19) in HeLa cells by Immunofluorescence.
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![Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)](https://www.abcam.com/ps/products/109/ab109462/Images/ab109462-4-benefits-of-recombinant-antibodies.png)
Anti-PTS/PTPS (phospho S19) antibody [EPR1517(2)] (ab109462)
