Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11890] to PSIP1/LEDGF
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-PSIP1/LEDGF antibody [EPR11890]
    See all PSIP1/LEDGF primary antibodies

  • Description

    Rabbit monoclonal [EPR11890] to PSIP1/LEDGF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PSIP1/LEDGF aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O75475

  • Positive control

    • WB: HEK293T, Jurkat, HeLa and K562 cell lysates. IHC-P: Human brain and hepatocellular carcinoma tissues. ICC/IF: K562 cells.

  • General notes

     This product was previously labelled as PSIP1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
    Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
    All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : PSIP1 knockout HEK293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 60 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Lanes 1-3: Merged signal (red and green). Green - ab177159 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab177159 Anti-PSIP1/LEDGF antibody [EPR11890] was shown to specifically react with PSIP1/LEDGF in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266464 (knockout cell lysate ab258144) was used. Wild-type and PSIP1/LEDGF knockout samples were subjected to SDS-PAGE. ab177159 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
    Immunocytochemistry/ Immunofluorescence - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

    Immunofluorescent analysis of K562 cells labeling PSIP1/LEDGF with ab177159 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PSIP1/LEDGF with ab177159 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
    Western blot - Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
    All lanes : Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 60 kDa

  • Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)
    Anti-PSIP1/LEDGF antibody [EPR11890] (ab177159)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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