Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 02, 2021

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]
See all pro Caspase-1 + p10 + p12 primary antibodies
Description

Rabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Untreated THP-1 and treated with 80nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysates; Rat lung and spleen lysates; Human fetal lung lysates; J774A.1 and RAW 264.7 whole cell lysates. IP: RAW 264.7 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16883
Isotype

IgG
Research areas

Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia cell line) treated with 80nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 45, 42, 35, 12, 10 kDa
Observed band size: 10,12,35,42,45 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20 and p10(p12) subunits. PMID: 8662843. The product recognizes p10(p12) cleavage fragment where the immunogen is located.
Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

Lane 1 : Rat lung lysate
Lane 2 : Human fetal lung lysate
Lane 3 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
Lane 4 : Rat spleen lysate
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 45, 42, 35, 12, 10 kDa
Observed band size: 42,45 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1: 30 seconds; Lane 2,3 and 4: 10 seconds; Lane 5: 8 seconds.
Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.

Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

Lane 2: ab179515 IP in RAW 264.7 whole cell lysate.

Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab179515 in RAW 264.7 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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