Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883]
    See all pro Caspase-1 + p10 + p12 primary antibodies

  • Description

    Rabbit monoclonal [EPR16883] to pro Caspase-1 + p10 + p12

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated THP-1 and treated with 80nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysates; Rat lung and spleen lysates; Human fetal lung lysates; J774A.1 and RAW 264.7 whole cell lysates. IP: RAW 264.7 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16883

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)
    Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)
    All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysate
    Lane 2 : THP-1 (Human monocytic leukemia cell line) treated with 80nM TPA overnight, then treated with 1 µg/ml LPS for 8 hours whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45, 42, 35, 12, 10 kDa
    Observed band size: 10,12,35,42,45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    There are six pro-caspase 1 isoforms, generating products between 30-45kDa, Pro-Caspase 1 is cleaved into p20 and p10(p12) subunits. PMID: 8662843. The product recognizes p10(p12) cleavage fragment where the immunogen is located.

  • Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)
    Western blot - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)
    All lanes : Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515) at 1/1000 dilution

    Lane 1 : Rat lung lysate
    Lane 2 : Human fetal lung lysate
    Lane 3 : J774A.1 (Mouse macrophage reticulum cell sarcoma cell line) whole cell lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45, 42, 35, 12, 10 kDa
    Observed band size: 42,45 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 30 seconds; Lane 2,3 and 4: 10 seconds; Lane 5: 8 seconds.

  • Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)
    Immunoprecipitation - Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

    pro Caspase1 + p10 + p12 was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab179515 at 1/100 dilution.

    Western blot was performed from the immunoprecipitate using ab179515 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

    Lane 2: ab179515 IP in RAW 264.7 whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of  ab179515 in RAW 264.7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)
    Anti-pro Caspase-1 + p10 + p12 antibody [EPR16883] (ab179515)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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