Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2649Y] to PRKAR2B - BSA and Azide free
  • Suitable for: IHC-P, ICC, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free
    See all PRKAR2B primary antibodies

  • Description

    Rabbit monoclonal [EP2649Y] to PRKAR2B - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, IP, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Rat, Mouse, and Human fat tissue sections. IP: Human brain lysate. ICC: Neuro-2a cells. WB: Human, mouse and rat brain lysate

  • General notes

    ab239870 is the carrier-free version of ab75993.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP2649Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    Western blot - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    All lanes : Anti-PRKAR2B antibody [EP2649Y] (ab75993) at 1/1000 dilution

    Lane 1 : Human brain lysate at 15 µg
    Lane 2 : Mouse brain lysate at 20 µg
    Lane 3 : Rat brain lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab75993, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

    This data was developed using ab75993, the same antibody clone in a different buffer formulation.
    Purified ab75993 at 1/30 dilution (2µg) immunoprecipitating PRKAR2B in Human brain lysate.
    Lane 1 (input): Human brain lysate 10µg
    Lane 2 (+): ab75993 + Human brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75993 in Human brain lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    This data was developed using ab75993, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    Immunocytochemistry - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    This data was developed using ab75993, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling PRKAR2B with purified ab75993 at 1/50 dilution (8.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    This data was developed using ab75993, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    This data was developed using ab75993, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)
    Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

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