Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2649Y] to PRKAR2B - BSA and Azide free
Suitable for: IHC-P, ICC, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free
See all PRKAR2B primary antibodies
Description

Rabbit monoclonal [EP2649Y] to PRKAR2B - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, ICC, IP, WBmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Rat, Mouse, and Human fat tissue sections. IP: Human brain lysate. ICC: Neuro-2a cells. WB: Human, mouse and rat brain lysate
General notes
ab239870 is the carrier-free version of ab75993.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2649Y
Isotype

IgG
Research areas

Western blot - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

All lanes : Anti-PRKAR2B antibody [EP2649Y] (ab75993) at 1/1000 dilution

Lane 1 : Human brain lysate at 15 µg
Lane 2 : Mouse brain lysate at 20 µg
Lane 3 : Rat brain lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 46 kDa
Observed band size: 52 kDa
why is the actual band size different from the predicted?

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Purified ab75993 at 1/30 dilution (2µg) immunoprecipitating PRKAR2B in Human brain lysate.
Lane 1 (input): Human brain lysate 10µg
Lane 2 (+): ab75993 + Human brain lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75993 in Human brain lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling PRKAR2B with purified ab75993 at 1/50 dilution (8.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

This data was developed using ab75993, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Anti-PRKAR2B antibody [EP2649Y] - BSA and Azide free (ab239870)

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