Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRKAR2B antibody [EP2649Y] (ab75993)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2649Y] to PRKAR2B
  • Suitable for: WB, IHC-P, ICC, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRKAR2B antibody [EP2649Y]
    See all PRKAR2B primary antibodies

  • Description

    Rabbit monoclonal [EP2649Y] to PRKAR2B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Mouse
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PRKAR2B (C terminal). The exact sequence is proprietary.

  • Positive control

    • IHC-P: Rat, Mouse, and Human fat tissue sections. IP: Human brain lysate. ICC: Neuro-2a cells. WB: Human, mouse and rat brain lysate

  • General notes

     This product was previously labelled as PKA 2 beta (regulatory subunit)

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP2649Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Western blot - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Lanes 1-2 : Anti-PRKAR2B antibody [EP2649Y] (ab75993) at 1/1000 dilution (Purified)
    Lane 3 : Anti-PRKAR2B antibody [EP2649Y] (ab75993) at 1/1000 dilution

    Lane 1 : Human brain lysate at 15 µg
    Lane 2 : Mouse brain lysate at 20 µg
    Lane 3 : Rat brain lysate at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunoprecipitation - Anti-PRKAR2B antibody [EP2649Y] (ab75993)

    Purified ab75993 at 1/30 dilution (2µg) immunoprecipitating PRKAR2B in Human brain lysate.
    Lane 1 (input): Human brain lysate 10µg
    Lane 2 (+): ab75993 + Human brain lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab75993 in Human brain lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunocytochemistry - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling PRKAR2B with purified ab75993 at 1/50 dilution (8.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse fat tissue sections labeling PRKAR2B with purified ab75993 at 1/1500 dilution (0.27 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-PRKAR2B antibody [EP2649Y] (ab75993)
    Anti-PRKAR2B antibody [EP2649Y] (ab75993)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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