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Anti-PRKAR2B antibody [EP2648] (ab75996)

Key features and details

  • Rabbit monoclonal [EP2648] to PRKAR2B
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PRKAR2B antibody [EP2648]
    See all PRKAR2B primary antibodies

  • Description

    Rabbit monoclonal [EP2648] to PRKAR2B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Human PRKAR2B aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • Fetal brain, human testis and NIH/3T3 cell lysates. Human brain tissue.

  • General notes

     This product was previously labelled as PKA 2 beta (regulatory subunit)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP2648

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PRKAR2B antibody [EP2648] (ab75996)
    Western blot - Anti-PRKAR2B antibody [EP2648] (ab75996)
    All lanes : Anti-PRKAR2B antibody [EP2648] (ab75996) at 1/20000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Human testis lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Flow Cytometry - Anti-PRKAR2B antibody [EP2648] (ab75996)
    Flow Cytometry - Anti-PRKAR2B antibody [EP2648] (ab75996)
    Overlay histogram showing SHSY-5Y cells stained with ab75996 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75996, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] (ab75996)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR2B antibody [EP2648] (ab75996)

    ab75996, at 1/100 dilution, staining PRKAR2B in paraffin-embedded human brain tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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