Anti-Prion protein PrP antibody [8H4] (ab61409)
![Anti-Prion protein PrP antibody [8H4] (ab61409)](https://www.abcam.com/ps/products/61/ab61409/Images/ab61409-448149-anti-prion-protein-prp-antibody-western-blot-brain-mouse.jpg "Anti-Prion protein PrP antibody [8H4] (ab61409)")
Key features and details
- Mouse monoclonal [8H4] to Prion protein PrP
- Suitable for: IP, Indirect ELISA, WB, ICC, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Sheep, Cow, Human, Monkey
- Isotype: IgG2b
Overview
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Product name
Anti-Prion protein PrP antibody [8H4]
See all Prion protein PrP primary antibodies -
Description
Mouse monoclonal [8H4] to Prion protein PrP -
Host species
Mouse -
Tested applications
Suitable for: IP, Indirect ELISA, WB, ICC, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Cow, Human, Monkey -
Immunogen
Recombinant full length protein corresponding to Mouse Prion protein PrP.
Database link: P04156 -
Epitope
The antibody epitope resides within amino acids 145-180 of human Prion protein PrP. -
Positive control
- Mouse brain extract
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Purified Immunoglobulin -
Clonality
Monoclonal -
Clone number
8H4 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Prion protein PrP antibody [8H4] (ab61409)Anti-Prion protein PrP antibody [8H4] (ab61409) at 4 µg/ml + Mouse brain extract
Secondary
Goat Anti-Mouse IgG-Peroxidase
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![Western blot - Anti-Prion protein PrP antibody [8H4] (ab61409)](https://www.abcam.com/ps/products/61/ab61409/Images/ab61409-233276-anti-prion-protein-prp-antibody-8h4-western-blot.jpg)
Western blot - Anti-Prion protein PrP antibody [8H4] (ab61409) This image is courtesy of an anonymous AbreviewAll lanes : Anti-Prion protein PrP antibody [8H4] (ab61409) at 1/2500 dilution
Lane 1 : Wild type mouse hippocampus whole tissue lysate
Lane 2 : Prnp-/- mouse hippocampus whole tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated sheep anti-mouse IgG monoclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 25-37 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
