Anti-PRDM1/Blimp1 antibody (ab106766)
Key features and details
- Goat polyclonal to PRDM1/Blimp1
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRDM1/Blimp1 antibody
See all PRDM1/Blimp1 primary antibodies -
Description
Goat polyclonal to PRDM1/Blimp1 -
Host species
Goat -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
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Immunogen
Synthetic peptide:
C-DISDNADRLEDVED
, corresponding to internal sequence amino acids 734-747 of Human PRDM1/Blimp1 (NP_001189.2) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRDM1/Blimp1 antibody (ab106766)All lanes : Anti-PRDM1/Blimp1 antibody (ab106766) at 0.1 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 nuclear lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 92 kDa
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Flow Cytometry - Anti-PRDM1/Blimp1 antibody (ab106766)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab106766 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody (ab106766)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab106766 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Western blot - Anti-PRDM1/Blimp1 antibody (ab106766)Anti-PRDM1/Blimp1 antibody (ab106766) at 0.3 µg/ml + A431 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
