Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16655] to PRDM1/Blimp1
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PRDM1/Blimp1 antibody [EPR16655]
    See all PRDM1/Blimp1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16655] to PRDM1/Blimp1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human diffuse large B cell lymphoma, tonsil and cerebral cortex tissues. IF: RAMOS cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

    Immunohistochemical analysis of paraffin-embedded Human diffuse large B cell lymphoma tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human diffuse large B cell lymphoma tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human tonsil tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling PRDM1/Blimp1 using ab198287 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
    Immunocytochemistry/ Immunofluorescence - Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAMOS (Human Burkitt's lymphoma cell line) cells, labeling PRDM1/Blimp1 with ab198287 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear and weakly cytoplasm staining on RAMOS cell line was observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab198287 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)
    Anti-PRDM1/Blimp1 antibody [EPR16655] (ab198287)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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