Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481) - BSA and Azide free
  • Suitable for: ICC/IF, Dot blot, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free
    See all PRC1 primary antibodies

  • Description

    Rabbit monoclonal [EP1514Y] to PRC1 (phospho T481) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects PRC1 phosphorylated at Theonine 481.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247354 is the carrier-free version of ab62366 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247354 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EP1514Y

  • Isotype

    IgG

  • Research areas

Images

  • Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
    Dot Blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
    This data was developed using ab62366, the same antibody clone in a different buffer formulation.Primary ab dilution 1:1000 dilution. Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Lane 1: PRC1 (pT481) phospho peptide Lane 2: PRC1 non-phospho peptide. Exposure time 3 minutes
  • Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
    Western blot - Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
    All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution

    Lane 1 : HeLa cell lysates (untreated)
    Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP labeled, at 1/2000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    This data was developed using ab62366, the same antibody clone in a different buffer formulation.

  • Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)
    Anti-PRC1 (phospho T481) antibody [EP1514Y] - BSA and Azide free (ab247354)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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