Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10401] to PPOX - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PPOX antibody [EPR10401] - BSA and Azide free
    See all PPOX primary antibodies

  • Description

    Rabbit monoclonal [EPR10401] to PPOX - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249564 is the carrier-free version of ab170884. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249564 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)

    This data was developed using ab170884, the same antibody clone in a different buffer formulation.

    ab170884 showing +ve staining in Human normal brain tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)

    This data was developed using ab170884, the same antibody clone in a different buffer formulation.

    ab170884 showing +ve staining in Human cervical carcinoma tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)

    This data was developed using ab170884, the same antibody clone in a different buffer formulation.

    ab170884 showing +ve staining in Human normal kidney tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)

    This data was developed using ab170884, the same antibody clone in a different buffer formulation.

    ab170884 showing +ve staining in Human thyroid gland carcinoma tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)

    This data was developed using ab170884, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PPOX using ab170884 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)
    Western blot - Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)
    Lane 1 : Anti-PPOX antibody [EPR10401] (ab170884) at 1/1000 dilution
    Lanes 2-4 : Anti-cardiac Troponin I antibody (ab1000) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HT1080 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 51 kDa



    This data was developed using ab170884, the same antibody clone in a different buffer formulation.

     

  • Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)
    Anti-PPOX antibody [EPR10401] - BSA and Azide free (ab249564)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.