Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
![Anti-PPAR gamma antibody [EPR23297-111] (ab272718)](https://www.abcam.com/ps/products/272/ab272718/Images/ab272718-395302-anti-ppar-gamma-antibody-epr23297111-western-blot-rat-ovary-tissue-lysate-20-ug.jpg "Anti-PPAR gamma antibody [EPR23297-111] (ab272718)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23297-111] to PPAR gamma
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PPAR gamma antibody [EPR23297-111]
See all PPAR gamma primary antibodies -
Description
Rabbit monoclonal [EPR23297-111] to PPAR gamma -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human adipose and ovary tissue lysate; Rat ovary tissue lysate; Mouse ovary tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23297-111 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)All lanes : Anti-PPAR gamma antibody [EPR23297-111] (ab272718) at 1/1000 dilution
Lane 1 : Rat ovary tissue lysate
Lane 2 : Mouse ovary tissue lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID:12023027).
Exposure time: 3 minutes.
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![Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)](https://www.abcam.com/ps/products/272/ab272718/Images/ab272718-395301-anti-ppar-gamma-antibody-epr23297111-western-blot-human-adipose-tissue-lysate-20-ug.jpg)
Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)All lanes : Anti-PPAR gamma antibody [EPR23297-111] (ab272718) at 1/1000 dilution
Lane 1 : Human adipose tissue lysate
Lane 2 : Human ovary tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 59 seconds.
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![Anti-PPAR gamma antibody [EPR23297-111] (ab272718)](https://www.abcam.com/ps/products/272/ab272718/Images/ab272718-3-benefits-of-recombinant-antibodies.png)
Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
