Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPAR gamma antibody [EPR23297-111] (ab272718)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23297-111] to PPAR gamma
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPAR gamma antibody [EPR23297-111]
    See all PPAR gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR23297-111] to PPAR gamma

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human adipose and ovary tissue lysate; Rat ovary tissue lysate; Mouse ovary tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
    Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
    All lanes : Anti-PPAR gamma antibody [EPR23297-111] (ab272718) at 1/1000 dilution

    Lane 1 : Rat ovary tissue lysate
    Lane 2 : Mouse ovary tissue lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 57 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: NIH/3T3 (PMID:12023027).

    Exposure time: 3 minutes.

  • Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
    Western blot - Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
    All lanes : Anti-PPAR gamma antibody [EPR23297-111] (ab272718) at 1/1000 dilution

    Lane 1 : Human adipose tissue lysate
    Lane 2 : Human ovary tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 59 seconds.

  • Anti-PPAR gamma antibody [EPR23297-111] (ab272718)
    Anti-PPAR gamma antibody [EPR23297-111] (ab272718)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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