Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)
![Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)](https://www.abcam.com/ps/products/185/ab185209/Images/ab185209-218507-anti-pp11-antibody-epr15137-n-terminal-western-blot.jpg "Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15137] to PP11 - N-terminal
- Suitable for: IP, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PP11 antibody [EPR15137] - N-terminal
See all PP11 primary antibodies -
Description
Rabbit monoclonal [EPR15137] to PP11 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human placenta, skin and fetal skin lysates; Human placenta tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15137 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)All lanes : Anti-PP11 antibody [EPR15137] - N-terminal (ab185209) at 1/10000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 47 kDaBased on the sequence analysis, ab185209 recognizes two isoforms with the predicted MWs of 42KDa and 47KDa, respectively.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)](https://www.abcam.com/ps/products/185/ab185209/Images/ab185209-218505-anti-pp11-antibody-epr15137-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)Immunohistochemical analysis of Paraffin-embedded Human placenta tissue labeling PP11 with ab185209 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)](https://www.abcam.com/ps/products/185/ab185209/Images/ab185209-218506-anti-pp11-antibody-epr15137-n-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)Western blot analysis of immunoprecipitation pellet from Human fetal skin lysate immunoprecipitated using ab185209 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)](https://www.abcam.com/ps/products/185/ab185209/Images/ab185209-4-benefits-of-recombinant-antibodies.png)
Anti-PP11 antibody [EPR15137] - N-terminal (ab185209)
