Anti-PORCN/PPN antibody [EPR19741] - BSA and Azide free (ab251355)
![Anti-PORCN/PPN antibody [EPR19741] - BSA and Azide free (ab251355)](https://www.abcam.com/ps/products/251/ab251355/Images/ab251355-399167-anti-porcn-ppn-antibody-epr19741-bsa-and-azide-free-western-blot.jpg "Anti-PORCN/PPN antibody [EPR19741] - BSA and Azide free (ab251355)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19741] to PORCN/PPN - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-PORCN/PPN antibody [EPR19741] - BSA and Azide free
See all PORCN/PPN primary antibodies -
Description
Rabbit monoclonal [EPR19741] to PORCN/PPN - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251355 is the carrier-free version of ab201793 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab251355 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as PORCN
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19741 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PORCN/PPN antibody [EPR19741] - BSA and Azide free (ab251355)All lanes : Anti-PORCN/PPN antibody [EPR19741] (ab201793) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab201793, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Our immunogen covers all 5 isoforms but only isoform 5 (42kDa) is observed in our testing condition. This is consistent with the literature (PMID: 21768372).
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![Anti-PORCN/PPN antibody [EPR19741] - BSA and Azide free (ab251355)](https://www.abcam.com/ps/products/251/ab251355/Images/ab251355-2-benefits-of-recombinant-antibodies.png)
Anti-PORCN/PPN antibody [EPR19741] - BSA and Azide free (ab251355)