Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23314-256] to Polymeric immunoglobulin receptor/PIGR - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free
    See all Polymeric immunoglobulin receptor/PIGR primary antibodies

  • Description

    Rabbit monoclonal [EPR23314-256] to Polymeric immunoglobulin receptor/PIGR - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human colon and colon cancer tissue lysates; His-tagged recombinant human Polymeric immunoglobulin receptor/PIGR protein. IHC-P: Human colon, colon carcinoma and kidney tissue. IP: Human colon tissue lysate.

  • General notes

    This antibody (ab275030, clone EPR23314-256) is a different clone to ab273512 (clone EPR23314-78).

    ab275030 is the carrier-free version of ab275020. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23314-256

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)

    This data was developed using ab275020, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colon carcinoma (PMID: 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    All lanes : Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution

    Lane 1 : Human colon tissue lysate
    Lane 2 : Human colon cancer tissue lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 85-100 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275020, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Low expression cell line: A549 (PMID:21965228).

  • Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)

    This data was developed using ab275020, the same antibody clone in a different buffer formulation.

    Polymeric immunoglobulin receptor/PIGR was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab275020 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275020 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human colon tissue lysate 10 ug

    Lane 2: ab275020 IP in Human colon tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275020 in human colon tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)

    This data was developed using ab275020, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colonic mucosa (PMID: 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution + His-tagged recombinant human Polymeric immunoglobulin receptor/PIGR protein 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa



    This data was developed using ab275020, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer:5% NFDM/TBST.

    Exposure time: 6 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)

    This data was developed using ab275020, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human kidney (PMID: 31404540). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)
    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] - BSA and Azide free (ab275030)

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