Human PD-L2 ELISA Kit (ab231928)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.7 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PD-L2 ELISA Kit
See all PD-L2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.9% Inter-assay Sample n Mean SD CV% Serum 3 6% -
Sample type
Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.7 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 102 101% - 103% Milk 82 77% - 85% Urine 97 92% - 100% Serum 103 101% - 104% Cell culture media 99 99% - 99% Hep Plasma 100 95% - 107% EDTA Plasma 92 89% - 96% Cit plasma 100 92% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human PD-L2 ELISA Kit (ab231928) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-L2 protein in edta plasma, hep plasma, milk, saliva, serum, urine, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PD-L2 with 2.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PD-L2 Capture Antibody 1 x 600µl 10X Human PD-L2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human PD-L2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. -
Tissue specificity
Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted; Cell membrane and Endomembrane system. - Information by UniProt
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Alternative names
- B7 dendritic cell molecule
- B7-DC
- B7DC
see all -
Database links
- Entrez Gene: 80380 Human
- Omim: 605723 Human
- SwissProt: Q9BQ51 Human
- Unigene: 532279 Human
- Unigene: 617872 Human
Images
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Other - Human PD-L2 ELISA Kit (ab231928)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human PD-L2 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PD-L2 in human serum and plasma samplesThe concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 10%, plasma (EDTA) 5%, and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 9.7 ng/mL in serum, 11.1 ng/mL in plasma (citrate), 7.6 ng/mL in plasma (EDTA) and 8.5 ng/mL in plasma (heparin).
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 10.3 ng/mL with a range of 7.5 – 15.0 ng/mL.
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Interpolated concentrations of native PD-L2 in human urine and milk samplesThe concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50% and milk 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 2172 pg/mL in urine and 151 pg/mL in milk.
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Interpolated concentrations of spiked PD-L2 in human saliva and cell culture media samplesThe concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native PD-L2 in human PBMC cell culture supernatant samples stimulated or unstimulated with PHA/PMAThe concentrations of PD-L2 were measured in duplicates, interpolated from the PD-L2 standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L2 concentration was determined to be 32 pg/mL in stimulated PBMC cell culture supernatant and undetectable in unstimulated and media controls.
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Sandwich ELISA - Human PD-L2 ELISA Kit (ab231928)To learn more about the advantages of recombinant antibodies see here.