Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23314-78] to Polymeric immunoglobulin receptor/PIGR
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78]
    See all Polymeric immunoglobulin receptor/PIGR primary antibodies

  • Description

    Rabbit monoclonal [EPR23314-78] to Polymeric immunoglobulin receptor/PIGR

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged recombinant human pIgR protein; Human stomach, colon, colon cancer and lung tissue lysates. IP: Human stomach tissue lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23314-78

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)
    Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)
    All lanes : Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728) at 1/1000 dilution

    Lane 1 : Human stomach tissue lysate
    Lane 2 : Human colon tissue lysate
    Lane 3 : Human colon cancer tissue lysate
    Lane 4 : Human lung tissue lysate
    Lane 5 : A549 (human lung carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 85-100 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression cell line: A549 (PMID:21965228).

    Exposure times: Lane 1: 3 minutes; Lane 2: 10 seconds; Lane 3: 3 seconds; Lanes 4-5: 59 seconds.  

  • Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)
    Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)

    Polymeric immunoglobulin receptor/PIGR was immunoprecipitated from 0.35 mg Human stomach tissue lysate with ab272728 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 272728 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Human stomach tissue lysate 10 ug

    Lane 2: ab272728 IP in Human stomach tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272728 in Human stomach tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 24 seconds

     

  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)
    Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)
    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728) at 1/1000 dilution + His-tagged Recombinant Human pIgR protein 10 ng

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa
    Observed band size: 100 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds.

  • Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)
    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-78] (ab272728)

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