Anti-POLR1D antibody (ab243591)
Key features and details
- Rabbit polyclonal to POLR1D
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLR1D antibody
See all POLR1D primary antibodies -
Description
Rabbit polyclonal to POLR1D -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human POLR1D aa 1 to the C-terminus.
Database link: P0DPB5 -
Positive control
- IHC-P: Human pancreas tissue. WB: POLR1D over-expression lysate HEK- 293T cell lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa). ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Rabbit Polyclonal to POLR1D (ab243591)All lanes : Anti-POLR1D antibody (ab243591) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cell lysate (human epithelial cell line from embryonic kidney transformed with large T antigen)
Lane 2 : POLR1D over-expression HEK-293T cell lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 14 kDa
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Immunocytochemistry/ Immunofluorescence - Rabbit Polyclonal to POLR1D (ab243591)4% paraformaldehyde-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling POLR1D (Green) using ab243591 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Polyclonal to POLR1D (ab243591)Paraffin-embedded human pancreas tissue stained for POLR1D using ab243591 at 1/200 dilution in immunohistochemical analysis.
