Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17298] to PMF-1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PMF-1 antibody [EPR17298] - BSA and Azide free
    See all PMF-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17298] to PMF-1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251280 is the carrier-free version of ab199531. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251280 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
    Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
    All lanes : Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/1000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab199531, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
    Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
    All lanes : Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/5000 dilution

    Lane 1 : Human fetal brain tissue lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab199531, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
    Western blot - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
    All lanes : Anti-PMF-1 antibody [EPR17298] (ab199531) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa
    Observed band size: 26 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab199531, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
    Immunoprecipitation - Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)

    This data was developed using ab199531, the same antibody clone in a different buffer formulation.

    PMF-1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179466 at 1/80 (or 5 µg). Western blot was performed using ab199531 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HeLa whole cell extract at 10ug (input)

    Lane 2: HeLa whole cell extract PMF-1 immunoprecipitate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199531 in HeLa whole cell extract immunoprecipitation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)
    Anti-PMF-1 antibody [EPR17298] - BSA and Azide free (ab251280)

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